Unusual genetic mutation linked to adolescent liver cancer


February 28, 2014
Health

In the race for better treatments and possible cures, rare diseases are often left behind. In a collaboration of researchers at The Rockefeller University, Memorial Sloan Kettering Cancer Center and the New York Genome Center (NYGC), an unusual mutation has been found that is strongly linked to one such disease: a rare liver cancer that affects teens and young adults. The results, published this week in Science, suggest that the mutation plays a key role in the development of the disease, called fibrolamellar hepatocellular carcinoma, and may also underlie more common cancers as well.

With only about 200 people diagnosed each year with fibrolamellar, the disease is as rare as it is mysterious — there are no known causes and it’s difficult to both detect and treat. By the time doctors discover a growing tumor it is often too late, and the cancer has spread.

“We reasoned that it would be easier to identify genetic mutations in the tumors of young patients than in older ones, because in older people, the genome has been altered by years of aging and environmental factors,” says Elana Simon, a co-first author on the study and a member of the Rockefeller team. “Both the person as a whole and the tumor itself will have had less time to accumulate mutations, so those that we do find are more likely to be relevant.”

The researchers sequenced DNA and RNA at NYGC from tumors that had been surgically removed from 15 people with fibrolamellar. NYGC computational biologists and members of the Simon Laboratory at The Rockefeller University discussed the possibility of using a series of computer algorithms to search for sequence differences between the tumor samples and samples of healthy liver tissue. One abnormality that really stood out, and it was present in all 15 patients: a piece of DNA that had been broken and rejoined, creating a mutated gene that had the potential to wreak havoc in the bodies of individuals with the gene.

“We discovered chimeric RNAs in the tumor samples — made when DNA deletions create unnatural products that can drive cancer,” says Nicolas Robine, co-first author and NYGC Computational Biologist.  “This chimera had never been seen before, so we believe it will help drive the work of our Rockefeller colleagues and Elana’s future.  It is the NYGC’s mission to undertake such collaborative genomic studies that will accelerate medical advances.”

“Because of the deletion and then rejoining of the DNA, a new gene that was a mixture of two previous genes was created, called a chimera,” says Elana Simon. “A number of other types of tumors have been shown to be driven by chimeras, but this one is unique — it codes for a kinase, an enzyme that modifies other proteins, that has not been identified in cancers.” Furthermore, the researchers found that the kinase was made only in the tumor cells, and that it was constantly active. They believe that overproduction of this kinase may explain the uncontrolled growth of the tumor.

“These results were extremely encouraging,” says Sanford Simon, the study’s senior author and head of Rockefeller’sLaboratory of Cellular Biophysics. “It is uncommon for a genetic screen for a cancer to turn up such a strong candidate mutation, and for the mutation to be present in every single patient tested.”

For Elana Simon, who is finishing her senior year in high school and did the work after school and during breaks, the results are not only a scientific success, but also a profoundly personal one: her interest in studying the disease developed after she herself was diagnosed with fibrolamellar six years ago. The study was conducted in collaboration with the surgeon who removed her tumor in 2008, Michael P. LaQuaglia, chief of the Pediatric Surgical Service at Memorial Sloan Kettering Cancer Center, and her father, Sanford Simon, as well as the NYGC team.

The research is also unusual in that it was funded not by a federal grant, but largely with private gifts from theFibrolamellar Cancer Foundation and several individual donors whose lives have been touched by the disease. Additional support was also provided by the Howard Hughes Medical Institute, the New York Genome Center, The Rockefeller University Center for Clinical and Translational Science and by a gift to The Rockefeller University by an anonymous donor.

The Simon lab is now working on testing the effects of the chimera on human liver cells and in mouse livers, to further elucidate its role in the disease. If they can understand what’s causing the tumors to develop, the scientists can work on not only treating them — using a genetic target to halt the cancer’s growth — but also catching them before they even appear.

“The hope is that we’d be able to screen the blood for the presence of this chimera, and patients wouldn’t have to wait until the tumors are present, until it might be too late, to do something about it,” says Sanford Simon.

The work may also have implications for other cancers. “Genomics is allowing us to classify cancers based not on the organ they originate in, but on the molecular changes they trigger,” says Sanford Simon. “Now that we know about this new chimera kinase, we can look for it in other cancers and work to develop new tools that will someday radically improve our ability to fight disease.”

“NYGC is thrilled to have this work be our first published example of the explosive power of collaborations between deeply invested biologists like those in the Simon lab including Elana Simon, and thoughtful bioinformatics scientists like Nicolas Robine and NYGC team, who worked together so effectively with the tools of genomic sequencing and analysis to discover this new chimeric protein and cancer target,” says Robert Darnell, head of Rockefeller’s Laboratory of Molecular Neuro-Oncology, HHMI investigator, and president and scientific director of NYGC. “The work done serves as an exemplar for how the power of interdisciplinary and inter-institutional genomic science has the potential to save people’s lives in New York and beyond.”Unusual genetic mutation linked to adolescent liver cancer



Unusual genetic mutation linked to adolescent liver cancer

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