May 25, 2014
In what is believed to be the largest and most detailed genetic analysis of its kind, researchers at NYU Langone Medical Center and elsewhere have concluded that 69 percent of healthy American adults are infected with one or more of 109 strains of human papillomavirus (HPV). Only four of the 103 men and women whose tissue DNA was publicly available through a government database had either of the two HPV types known to cause most cases of cervical cancer, some throat cancers, and genital warts.
Researchers say that while most of the viral strains so far appear to be harmless and can remain dormant for years, their overwhelming presence suggests a delicate balancing act for HPV infection in the body, in which many viral strains keep each other in check, preventing other strains from spreading out of control. Although infection is increasingly known to happen through skin-to-skin contact, HPV remains the most common sexually transmitted infection in the United States. It is so common that experts estimate nearly all men and women contract some strain of it during their lives.
“Our study offers initial and broad evidence of a seemingly ‘normal’ HPV viral biome in people that does not necessarily cause disease and that could very well mimic the highly varied bacterial environment in the body, or microbiome, which is key to maintaining good health,” says senior study investigator and NYU Langone pathologist Zhiheng Pei, MD, PhD. Dr. Pei, an associate professor at NYU Langone, plans to present his team’s findings on May 20 in Boston at the annual meeting of the American Society for Microbiology.
Lead study investigator and NYU Langone research scientist Yingfei Ma, PhD, says “the HPV ‘community’ in healthy people is surprisingly more vast and complex than previously thought, and much further monitoring and research is needed to determine how the various non-cancer-causing HPV genotypes interact with the cancer-causing strains, such as genotypes 16 and 18, and what causes these strains to trigger cancer.”
For the study, which took two years to complete, researchers analyzed data made publicly available from the National Institutes of Health (NIH) Human Microbiome Project, which is gathering information on microorganisms’ effects on human health.
The NIH data consisted of comprehensive DNA analyses assembled by a technique called shotgun sequencing. The DNA decoding technique helped sort through vast amounts of genetic material among 748 tissue swabs of study participants’ major organs, including skin, vagina, mouth, and gut. Tissue samples were originally collected from healthy study volunteers, ages 18 to 80, participating in the NIH project. In shotgun sequencing, the genetic code of long strands of DNA is deciphered in a random firing pattern, much like pixels in a photo, until a full picture becomes apparent.