Hershey scientists improve methods for analysis of healthful cocoa compounds

Two scientific publications report on improved methods for determining the amounts of flavanol antioxidants in cocoa and chocolate. The research, sponsored by The Hershey Center for Health and Nutrition, was a collaboration between scientists at The Hershey Company and other scientific laboratories.

Scientists at Planta Analytica (Danbury, CT) isolated and separated cocoa flavanol antioxidants on a large scale. The Hershey scientists and collaborating scientists at the Pennsylvania State University-M.S. Hershey Medical Center (Hershey, PA) teamed up to determine the purity of these flavanols by HPLC and by Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) mass spectroscopy. The isolated compounds were then used as standards in the determination of flavanol cocoa antioxidants in a cocoa powder and a dark chocolate.

“We believe this represents the first large scale purification of standards for flavanol antioxidant determination” said Dr. Jeffrey Hurst of the Hershey Center for Health and Nutrition. “Prior to this, only dimers were commercially available. With a full series of standards, our flavanol determinations are not only more accurate, but the values are much higher, between 40% to 100% higher, than previously published methods using proprietary standards.” This also means that standards are commercially available to various laboratories. This collaborative work was published in the online journal

In other research published in the Journal Association of Official Analytical Chemists, scientists from The Hershey Company and Brunswick Laboratories (Newton, MA) reported on the development of a new method for determining total procyanidins. This method is a colorimetric test based on the specific reaction of dimethylaminocinnamaldehyde (DMAC) with flavanols. The method measures flavanol monomers, including EGCG, as well as higher flavanol polymers. The method which is standardized using a commercially available flavanol dimer, was validated at two Brunswick Laboratories facilities and at Hershey with all three laboratories providing comparable results at the 95% confidence level. “The specific reaction of DMAC with the flavanols has been known since the 1950s. This method is a simple and quick way to measure total procyanidins in cocoa and chocolate” said Dr. Mark Payne of the Hershey Center for Health and Nutrition. “Compared to the HPLC method, which separates individual compounds, this method gives one number, which importantly includes polymers of flavanols beyond ten.”

“These reports are part of an ongoing series of publications, by Hershey and its collaborators, designed to improve upon the methods to determine flavanol antioxidants from cocoa and chocolate,” said Dr. David Stuart, of the Hershey Center for Health and Nutrition. “We want to make these methods generally available to the chocolate industry initially, with the intent of having uniformly agreed upon methods of determining the level of these important molecules.”

These new methods can be used in research and other applications involving dietary intake of cocoa and chocolate, clinical interventions and food standardization”.

About The Hershey Company

The Hershey Company (NYSE: HSY) is the largest producer of quality chocolate in North America and a global leader in chocolate and sugar confectionery. Headquartered in Hershey, Pa., The Hershey Company has operations throughout the world and more than 12,000 employees. With revenues of more than $5 billion, Hershey offers such iconic brands as Hershey’s, Reese’s, Hershey’s Kisses, Kit Kat, Twizzlers and Ice Breakers as well as the smooth, creamy indulgence of Hershey’s Bliss chocolates. Hershey is a leader in the fast-growing dark and premium chocolate segment, with such brands as Hershey’s Special Dark and Hershey’s Extra Dark. In addition, Artisan Confections Company, a wholly owned subsidiary of The Hershey Company, markets such premium chocolate offerings as Scharffen Berger and Dagoba. For more than 100 years, The Hershey Company has been a leader in making a positive difference in the communities where we live, work and do business. The Milton Hershey School, established by the company’s founder in 1909, provides a nurturing environment, quality education, housing, and medical care at no cost to children in social and financial need. The School is administered by the Hershey Trust Company, Hershey’s largest shareholder, making the students of Milton Hershey School direct beneficiaries of Hershey’s success. Please visit us at www.hersheys.com.

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