A University of Arizona College of Medicine – Phoenix research team has developed a blood self-collection device to quickly estimate a person’s exposure to radiation in the event of a nuclear accident or attack.
A study led by Jian Gu and scientists at the medical school’s Center for Applied NanoBioscience and Medicine reports development of a system for packaging critical components of a traditional blood-collection kit to create an integrated fingerstick blood collector for radiation countermeasures.
The study, “Development of an integrated fingerstick blood self-collection device for radiation countermeasures,” was published Oct. 16 in PLOS One, a peer-reviewed open-access journal that features research, commentary and analysis across all areas of science and medicine.
An easy-to-use, self-administered blood test that could quickly evaluate a person’s radiation exposure would help triage emergency medical treatment in the event of a radiological or nuclear event. The U.S. Department of Health and Human Services long has sought ways to monitor a population’s radiation exposure following such an event.
“Our research addressed a critical need of sample collection and pre-processing in biodosimetry logistics after a large-scale radiological event for radiation countermeasures,” Gu said.
Biological dosimetry determines the extent of DNA damage caused by ionizing radiation associated with acute exposure from a dirty bomb or nuclear accident. In ionizing radiation, electrons are knocked out of atoms and form charged particles.
In a nuclear event, hundreds of thousands of people would need to be screened in a very short time, and traditional medical infrastructure for blood collection may not be available.
The specimens need to be prepared for biodosimetry assays after collection. These assays measure the physical changes in a person’s tissues from radiation. Gu’s work concentrated on the process of collecting and preparing blood to test exposure levels by providing a device that allows a person to collect their own sample that automatically could mix with assay reagents to hasten the process.
Using 3D printing, a miniaturized vacuum tube, integrated capillaries and a lancet were fabricated into a self-collection device that can process the blood specimen for both cytogenetic and gene expression biodosimetry that then would be analyzed in a centralized bioanalytical laboratory. Cytogenetic biodosimetry measures the response of circulating blood lymphocytes in the body to accurately estimate the absorbed radiation dose. Gene expression biodosimetry measures the expression levels of a panel of radiation-sensitive genes for the absorbed dose. Results could be returned in one day in the gene expression tests and three days with the cytogenic tests.
The device was easy to use for people who never had used a fingerstick blood collector and delivered results similar to samples collected using traditional methods, Gu said. The integrated format avoided the possibility of contamination.
“The integrated collector will alleviate the sample collection bottleneck for radiation countermeasures following a large-scale nuclear event, and may be useful in other applications with its self-collection and liquid reagent sample preprocessing capabilities,” Gu said.
The study is part of a core National Institutes of Health program, led since 2005 by Frederic Zenhausern, director of the Center for Applied NanoBioscience and Medicine, in partnership with Columbia University and Georgetown University, under a U19 National Institute of Allergy and Infectious Diseases grant, sponsored by the Center for Medical Countermeasures Against Radiation.
Research funding was supported by NIH/NIAID grant No. 5U19AI067773.