Researchers from the UCLA Henry Samueli School of Engineering and Applied Science have developed a new cell phone–based fluorescent imaging and sensing platform that can detect the presence of the bacterium Escherichia coli in food and water. The engineers combined antibody functionalized glass capillaries with quantum dots (semiconductors often used for medical imaging) as signal reporters to specifically detect E. coliparticles in liquid samples using a lightweight, compact attachment to an existing cell-phone camera.
Using battery-powered, inexpensive light-emitting diodes (LEDs), the researchers can excite/pump labeled E. coli particles captured on the capillary surface; there, emissions from the quantum dots can be imaged with the cell-phone camera, using an additional lens inserted between the capillary and the cell phone.
E. coli can easily contaminate food and drinking water. It poses a significant threat to public health, even in highly developed parts of the world, and causes a large number of hospitalizations and deaths every year. As few as 10–100 E. coli particles can kill the cells of the intestinal lining, destroy the kidneys and cause blood clots in the brain, as well as seizures, paralysis and respiratory failure.
Authors of the research include UCLA electrical engineering postdoctoral scholar Hongying Zhu; UCLA electrical engineering undergraduate student Uzair Sikora; and UCLA associate professor of electrical engineering and bioengineering Aydogan Ozcan. Ozcan is also a member of the California NanoSystems Institute at UCLA. More on Ozcan’s research group can be found at http://innovate.ee.ucla.edu.
The Ozcan Research Group is funded by the U.S. Office of Naval Research, the National Institutes of Health, the National Science Foundation and the U.S. Army Research Office.
The research is published in the peer-reviewed journal The Royal Society of Chemistry and is available online at http://pubs.rsc.org/en/content/articlelanding/2012/an/c2an35071h.