Copy Number Determination Optimisation Using Real Time PCR Machine

The objectives of this study is to check what gene is suitable for creation of a standard,to establish real time PCR as a high throughput technique for copy number work and to optimize / prepare a good standard for absolute quantification analysis for this work.
We designed two strategies for the optimization of qRT-PCR. The first one is through Southern blot copy number correlation with qRT-PCR. The second is through the creation of standard using a plasmid carrying the construct that was used in the plant.
In lieu of the first objective to find a gene suitable for doing copy number work we encountered problem with NOS gene.

We did the serial dilution of the samples following the computation by Applied Biosystem.


The material in this press release comes from the originating research organization. Content may be edited for style and length. Want more? Sign up for our daily email.