Copy Number Determination Optimisation Using Real Time PCR Machine

The objectives of this study is to check what gene is suitable for creation of a standard,to establish real time PCR as a high throughput technique for copy number work and to optimize / prepare a good standard for absolute quantification analysis for this work.
We designed two strategies for the optimization of qRT-PCR. The first one is through Southern blot copy number correlation with qRT-PCR. The second is through the creation of standard using a plasmid carrying the construct that was used in the plant.
In lieu of the first objective to find a gene suitable for doing copy number work we encountered problem with NOS gene.

We did the serial dilution of the samples following the computation by Applied Biosystem.


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