HOUSTON — (June 17, 2009) — An overactive enzyme is behind a leaky calcium channel that plays a role in the development of atrial fibrillation, which is the most common cardiac arrhythmia that is responsible for a third of all strokes. However, it doesn’t act alone, say researchers at Baylor College of Medicine. The findings can be found online in the current edition of the Journal of Clinical Investigation.
“When the heart pumps properly, the muscle contractions are regulated by waves of calcium. When the heart relaxes, the calcium is stored; when the heart contracts, it is released,” said Dr Xander Wehrens, assistant professor of molecular physiology and biophysics, and cardiology at BCM. “In atrial fibrillation, the calcium is released too early. As it leaks out, the heart beats too fast and irregularly.”
Researchers knew that an enzyme called calmodulin kinase II is overactive in several heart diseases and believed it played a role in the leaky channels — a tiny pore that controls release of calciu through the cell’s membrane. In the current study, Wehrens and colleagues were able to show in mouse models that if this enzyme is inhibited, calcium channels normalize and atrial fibrillation is prevented.
To determine if the calcium leak alone was enough to set off atrial fibrillation, researchers bred a mouse with a specific genetic mutation in the calcium channel, making it prone to leaks.
“The mice were fine. They did not develop atrial fibrillation despite the leak,” Wehrens said. “There had to be another factor that contributed to arrhythmias.”
Wehrens said they discovered that a sudden increase in heart rate is a very specific activator of calmodulin kinase II. The research team found that mice with the mutation were more prone to atrial fibrillation after their heart rate was sped up, activating the enzyme. Mice that lacked the mutation did not suffer from arrhythmias despite having the enzyme activated. From that they surmised that the enzyme alone did not lead to atrial fibrillation.
When they used a drug to block activity calmodulin kinase II, and the mices’ heart rates were raised, the mice with the calcium channel mutation had no signs of arrhythmia. Again the results further supported the conclusion that the enzyme does play a role in the disorder but does not act alone.
“It’s a synergy. The models had to have a preexisting problem on top of overactive calmodulin kinase II,” Wehrens said. “If you don’t have the mutation and the enzyme, but only one or the other, then you don’t develop the arrhythmia.”
Since calmodulin kinase II is important to many other functions in the heart, blocking it all together is not a realistic treatment. Instead, they were able to make a change to one amino acid in the genetic code and stop the specific calcium channel from being affected by the enzyme. Further studies with similar models showed this as an effective treatment.
“More trials are needed, but this is a promising way to target one regulatory event that contributes to atrial fibrillation,” Wehrens said. “These findings could lead to new drug therapies for arrhythmias and better patient care.”
Others who took part in the study include Drs. Mihail Chelu, Satyam Sarma, Subeena Sood, Ralph van Oort, Darlene Skapura, Na Li, and Marco Santonastasi, all of the department of molecular physiology and biophysics at BCM; Sufen Wang and Miquel Valderrábano, both of the department of cardiology at The Methodist Hospital; Frank-Ulrich Müller and Wilhelm Schmitz, both of the Institute of Pharmacology and Toxicology, Universitätsklinikum Münster, Münster, Germany; Ulrich Schotten, department of physiology Maastricht University, Maastricht, The Netherlands; Mark E. Anderson, department of internal medicine, division of cardiovascular medicine, University of Iowa Carver College of Medicine; and Dobromir Dobrev, department of pharmacology and toxicology, Dresden University of Technology, Dresden, Germany.
Funding for the study came from the American Heart Association, the National Institutes of Health National Heart Lung and Blood Institute, the March of Dimes, the Heart Rhythm Society, the Houston Texans, and the Foundation Leducq Award to the Alliance of Calmodulin Kinase Signaling in Heart Disease.
The study can be found online at www.jci.org.
