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BGI Genomics

Figure S1. Study design, data analysis and experimental strategy for linking the human gut microbiota, genetics, and obesity. Related to STAR Methods. (A) Basic information of a Chinese obese cohort (n = 1,005). (B) Shotgun metagenomic sequencing and taxonomic profiling analysis on 1,005 collected fecal samples. (C) Whole genome sequencing conducted on 814 collected blood samples, and the calculation of polygenic risk score (PRS) for BMI based on BMI-associated common variants. (D) Identification of obesity-enriched microbial taxa, and assessment of the impacts of gut microbiota and host genetics on obesity. (E) Experimental design of functional examination of M. rupellensis and E. coli with heterologous expression of a myo-inositol-degrading enzyme in vitro and in vivo.

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